Dermatology
Issue
- Dermatology
- Autoimmune Blistering Skin Diseases
Personal Data
Dr. med. Silke Hofmann
Department
Molecular Dermatology, Department of Dermatology, University Medical Center Freiburg
Know-How
- Development of diagnostic ELISAs for detection of autoantibodies in patients sera (collagen XVII, collagen VII)
- Biochemical methods like immunoblot, immunoprecipitation, protein expression and purification
- Development of monoclonal antibodies
Selected Publications
1. Hofmann SC, Thoma-Uszynski S, Hunziker T, Bernard P, Koebnick C, Stauber A, Schuler G, Borradori L, Hertl M: Severity and phenotype of bullous pemphigoid relate to autoantibody profile against the NH2 – and COOH-terminal regions of the BP180 ectodomain. J Invest Dermatol 2002; 119: 1065-1073
2. Hofmann SC, Tamm K, Hertl M, Borradori L: Diagnostic value of an enzyme-linked immunosorbent assay using BP180 recombinant proteins in elderly patients with pruritic skin disorders. Br J Dermatol 2003; 149: 910-911
3. Seppänen A, Suuronen T, Hofmann SC, Majamaa K, Alafuzoff I: Distribution of collagen XVII in the human brain. Brain Res 2007; 1158:50-56.
4. Hofmann SC, Voith U, Sasaki T, Trüeb RM, Nischt R, Bruckner-Tuderman L: The autoantigen in anti-p200 pemphigoid is synthesized by keratinocytes and fibroblasts and is distinct from nidogen-2. J Invest Dermatol 2008; 128:87-95.
5. Hofmann SC, Technau K, Müller AMS, Lübbert M, Bruckner-Tuderman L: Bullous pemphigoid associated with hypereosinophilic syndrome: simultaneous response to imatinib.
J Am Acad Dermatol 2007; 56:S68-72.
Projects
- Characterization of the p200-autoantigen in anti-p200 pemphigoid
- Development and functional characterization of monoclonal antibodies against collagen XVII
- Analysis of blister fluids for the presence of collagen XVII
- Analysis of collagen XVII in human brain
- Antimicrobial peptides in Staph. aureus colonization and infection